Recipe

Blocking buffer for in situ hybridization (BBISH) (10X)

Reagent Quantity (for 10 mL) Final concentration (10X)
Blocking Reagent (Roche Applied Science 11096176001) 1 g 10%
Maleic acid buffer to 10 mL
Dissolve the Blocking Reagent in the maleic acid buffer with shaking and heating. Autoclave. Store aliquots at -20°C.

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  1. doi:10.1101/pdb.rec11525 Cold Spring Harb Protoc 2008: pdb.rec11525-
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