Recipe

Lysis buffer for sponge tissue

Reagent Quantity (for 50 mL) Final concentration
KCl (0.5 M) 5 mL 50 mM
Tris-Cl (1 M, pH 9.0) 0.5 mL 10 mM
Triton X-100 100 μL 0.1%
H2O to 50 mL
Store at room temperature.

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  1. doi:10.1101/pdb.rec11530 Cold Spring Harb Protoc 2008: pdb.rec11530-
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