Recipe

Alkaline phosphatase (AP) buffer

Reagent Quantity (for 100 mL) Final concentration
NaCl-Tris solution (5X) 20 mL 1X
MgCl2 (1 M) 5 mL 50 mM
Tween 20 (20%) 500 μL 0.1%
H2O to 100 mL
Filter (0.45 μm) before use. Prepare just before use.

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  1. doi:10.1101/pdb.rec11532 Cold Spring Harb Protoc 2008: pdb.rec11532-
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