Recipe

Trypsin-DNase solution

2 g trypsin (Worthington LS003667)

200 mL HHGN dissection solution

10 mL DNase (2 mg/mL)

Combine above reagents and adjust pH to 7.4 with NaOH. Filter-sterilize and divide into 5-mL aliquots. Store aliquots at −80°C.

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  1. doi:10.1101/pdb.rec11553 Cold Spring Harb Protoc 2008: pdb.rec11553-
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