Trypsin-DNase solution
2 g trypsin (Worthington LS003667)
200 mL HHGN dissection solution
Combine above reagents and adjust pH to 7.4 with NaOH. Filter-sterilize and divide into 5-mL aliquots. Store aliquots at −80°C.
2 g trypsin (Worthington LS003667)
200 mL HHGN dissection solution
Combine above reagents and adjust pH to 7.4 with NaOH. Filter-sterilize and divide into 5-mL aliquots. Store aliquots at −80°C.