Recipe

Salt gradient dialysis buffers

Reagent 2.5 mM NaCl 0.6 M NaCl 0.8 M NaCl 1.0 M NaCl
EDTA (0.5 M) 0.5 mL 0.5 mL 0.5 mL 0.5 mL
NaCl (5 M) 0.5 mL 120 mL 160 mL 200 mL
Tris-Cl (1 M, pH 8.0) 10 mL 10 mL 10 mL 10 mL
Adjust the volume of each solution to 1 L with H2O. Just before use add:
DTT (Dithiothreitol) (0.25 M) 2 mL 2 mL 2 mL 2 mL
Phenylmethylsulfonyl fluoride (0.1 M) 2 mL 2 mL 2 mL 2 mL

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  1. doi:10.1101/pdb.rec11584 Cold Spring Harb Protoc 2008: pdb.rec11584-
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