Table 1. Cryopreservation strategies

Table

Genotype of parents^a			Genotype of embryos		Minimum number of eight-cell embryos
Homozygous tg^b	×	Homozygous tg		100% homozygous tg	200
Homozygous tg	×	Heterozygous tg		50% homozygous tg, 50% heterozygous tg	200
Homozygous tg	×	Wild type		100% heterozygous tg	300
Heterozygous tg	×	Heterozygous tg		25% homozygous tg, 50% heterozygous tg, 25% wild type	450
Heterozygous tg	×	Wild type		50% heterozygous tg, 50% wild type	500

^a“Heterozygous” refers to both heterozygous and hemizygous genotypes.
^b(tg) Transgenic.