Table

Table 1. Developmental stages for Gryllus bimaculatus embryos

Stage Day hAEL Characteristics
1.0 ∼1 Freshly laid eggs are light yellow in color and 2.88 ± 0.12 mm long. The anterior pole of the egg is slightly pointed, and the posterior is blunt. The concave and convex sides are dorsal and ventral, respectively. The yolk is finely granular.
2.0 1 24 A uniform array of nuclei is visible on the surface of the yolk.
2.3 27 The bipartite germ anlage is formed.
2.4 28 Merging of the bipartite germ anlage from the posterior side begins.
2.6 30 The unitary germ anlage is formed.
3.0 30–36 The germ anlage in left-right direction contracts.
4.0 1.5 36 The germ band (from the head to the T2 segment) is formed.
4.3 39 The T3 segment is formed.
4.4 40 Elongation of the germ band to form the abdominal segments begins, as does wg expression in the A1 segment; A/E = 21%.
4.9 45 wg expression in the A2 segment begins; A/E = 28%.
5.0 46 wg expression in the A3 segment begins; A/E = 30%.
5.2 2 48 wg expression in the A4 segment begins; A/E = 40%.
6.0 2.5 60 Formation of the limb bud begins; A/E = 42%.
6.5 66 A/E = 44%.
7.0 3 72 wg expression in the A8/9 segments begins; A/E = 45%. The limb bud is formed without any segmentation boundary.
8.0 4 93 The boundary between the trochanter and femur is formed.
9.0 5 120 The boundary between the femur and tibia is formed.
10.0 5.5 132 The embryo undergoes katatrepsis. The tibial segment of the limb bud elongates.
11.0 6 144 After completion of katatrepsis, dorsal closure begins. The boundary between the tibia and tarsus is formed.
12.0 8 The femoral, tibial, and tarsal segments of the metathoracic leg are clearly distinguishable. At the distal end of the tibia, two pairs of spur primordia are generated. The tarsus is divided into three segments, and its distal end possesses the claw primordium.
13.0 9 Dorsal closure is completed. The embryo attains its full length and fills the entire egg. Cuticle secreted by the embryo covers the entire body. Pigmentation in the compound eye is visible. The legs begin to twitch. Rapid growth of the leg segments starts at this stage.
14.0 10 Each leg is fully grown. The tibial spurs and tarsal claws become sharp.
15.0 11 The embryo is light yellow in color. Regularly arranged black bristles appear on the leg and the posterior ridge of each tergum.
16.0 12–13 Most of the eggs are hatched.
(hAEL) Hours after egg-laying at 28°C. A/E = 100 × (length of the abdominal region)/(full length of the embryo).

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  1. doi:10.1101/pdb.tab1emo110 Cold Spring Harb Protoc 2008: pdb.tab1emo110-

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