Recipe

Spradling injection buffer

5 mM KCl

10 mM NaH2PO4 (pH 7.8)

To prepare this buffer, use a 100 mM stock solution of NaH2PO4.

Use 1-5 N NaOH to adjust the pH of the NaH2PO4 stock solution to 7.8.

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  1. doi:10.1101/pdb.rec11274 Cold Spring Harb Protoc 2008: pdb.rec11274-
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