Recipe

Hybridization solution (viscous)

Reagent Amount to add
Formamide (UltraPure) 5.0 mL
4 M NaCl 0.75 mL
1 M Tris-Cl (pH 7.5) 0.1 mL
20 mg/mL RNase-free yeast tRNA 50 μL
10% blocking reagent 0.75 mL
25% dextran sulfate 1 mL
0.5 M EDTA (pH 8) 50 μL
H2O (RNase-free) 2.3 mL

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  1. doi:10.1101/pdb.rec11288 Cold Spring Harb Protoc 2008: pdb.rec11288-
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