MEMFA
EGTA
Prepare a 10X stock solution of 1 M MOPS (pH 7.4), 20 mM EGTA, and 10 mM MgSO4.
On the day of use, prepare fresh 1X MEMFA by combining 8 parts H2O, 1 part 10X MEMFA stock solution, and 1 part formaldehyde (37%).
Final concentrations are 100 mM MOPS (pH 7.4), 2 mM EGTA, 1 mM MgSO4, 3.7% (v/v) formaldehyde.
