| 1 |
Signal intensity of each microarray spot |
Average of replicate signal intensities of channel 1 (e.g., Cy3) and channel 2 (e.g., Cy5) |
| 2 |
Signal intensity of each microarray spot |
Minimum signal intensity of channel 1 (e.g., Cy3) and channel 2 (e.g., Cy5) |
| 3 |
Difference between the signal intensity and background intensity of each microarray spot |
Average of the difference between the signal intensity and the background intensity of channel 1 (e.g., Cy3) and channel 2
(e.g., Cy5)
|
| 4 |
Difference between the signal intensity and background intensity of each microarray spot |
Minimum difference between the signal intensity and the background intensity of channel 1 (e.g., Cy3) and channel 2 (e.g.,
Cy5)
|
| 5 |
Ratio of signal intensity to background intensity of each spot |
Average of the ratio of the signal intensity to the background intensity of channel 1 (e.g., Cy3) and channel 2 (e.g., Cy5) |
| 6 |
Ratio of signal intensity to background intensity of each spot |
Minimum value of the ratio of the signal intensity to the background intensity of channel 1 (e.g., Cy3) and channel 2 (e.g.,
Cy5)
|
| 7 |
|
Reproducibility of the measurement value for each spot calculated as the difference between replicate experiments: log2(tarray1/carray1) - log2(tarray2/carray2), where tarray1 is the intensity of test data from microarray 1, carray1 is the corresponding control value of the spot from microarray 1, tarray2 is the intensity of test data from microarray 2, and carray2 is the corresponding control value of the spot from microarray 2
|
| 8 |
|
Relative expression value of each gene calculated as: log2(tarray/carray)
|
| 9 |
|
Overall reproducibility of the microarray (Pearson’s correlation coefficient of duplicate experiments) |
