Vital Stain for Plant Cytoplasm
This protocol was adapted from “How to Analyze a Mutant Phenotypically,” Chapter 4, in Arabidopsis (eds. Weigel and Glazebrook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.INTRODUCTION
This protocol describes a vital stain for plant cytoplasm. Fluorescein diacetate is used to identify viable cells, which will emit a greenish fluorescence. Dead cells will fluoresce red in the presence of propidium iodide, which does not enter live cells.
