Methyl cellulose solution

Heat H₂O to 60°C, add methyl cellulose (Sigma) (3% by weight; adjust viscosity if needed), and mix with a glass rod. Place the solution (including any undissolved particles) in a −20°C freezer and stir every 30 min until frozen. Store overnight in a refrigerator. The solution should be clear without visible specks of methyl cellulose. Store in 50-mL tubes in a larval incubator (28°C).