Recipe

IMAC wash buffer 2

8.3% (v/v) IMAC column elution buffer

91.7% (v/v) IMAC wash buffer 1

The final imidazole concentration of the solution should be 25 mM.

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  1. doi:10.1101/pdb.rec11321 Cold Spring Harb Protoc 2008: pdb.rec11321-
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