Table 1. Different methods to improve PNA delivery by peptide conjugation
| Conjugation strategy | Cell type | Target | Reference |
|---|---|---|---|
| CPP-S-S-PNA | Bowes melanoma cells | Galanin receptor 1 | Pooga et al. (1998) |
| CPP-PNA | Primary rat neurons | Pre-pro-oxytocin | Aldrian-Herrada et al. (1998) |
| NLS-PNA | COS-7, 3T3, and HeLa | PNA used for DNA delivery | Brandén et al. (1999) |
| CPP-S-S-NLS-PNA | Human prostate cancer cells | --- | Braun et al. (2002) |
| CPP-S-S-PNA-Decoy | Rinm5F | NF-κB through DNA decoy delivery | Fisher et al. (2004) El-Andaloussi et al. (2005) |
| Only the strategy for disulfide conjugation with CPP is described in this protocol, because this is a complicated but crucial step in many CPP-PNA applications. For other protocols, see the references. | |||
