Recipe

Phosphate/citrate buffer

0.1 M dibasic sodium phosphate

0.05 M sodium citrate (pH 5.0)

Adjust to pH 5.0 with phosphoric acid and filter sterilize (22-μm pore size filter). Prepare immediately before use.

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  1. doi:10.1101/pdb.rec11328 Cold Spring Harb Protoc 2008: pdb.rec11328-
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