Table 1. Common retrovirus packaging cell lines

Env protein source	Cell line name	Parental cell line	Marker gene(s) expressed^a	Source(s)	Reference
Ecotropic MLV	PE501	NIH-3T3 mouse	tk	Authors	Miller and Rosman (1989)
	GP+E-86	NIH-3T3 mouse	gpt	ATCC CRL-9642	Markowitz et al. (1988)
Amphotropic MLV	PA317	NIH-3T3 mouse	tk	ATCC CRL-9078	Miller and Buttimore (1986)
	ProPak-A	293 human	hpt, pac	ATCC CRL-12479	Forestell et al. (1997)
	FLYA	HT-1080 human	bsr, ble	Authors	Cosset et al. (1995)
10A1 MLV	PT67	NIH-3T3 mouse	tk, dhfr*	BD Biosciences, ATCC CRL-12284	Miller and Chen (1996)
GALV	PG13	NIH-3T3 mouse	tk, dhfr*	ATCC CRL-10686	Miller et al. (1991)
RD114	FLYRD	HT-1080 human	bsr, ble	Authors	Cosset et al. (1995)
^aMarker genes used during construction of packaging cells include herpes simplex virus thymidine kinase (tk), a mutant methotrexate-resistant dihydrofolate reductase (dhfr), xanthine-guanine phosphoribosyltransferase (gpt), hygromycin phosphotransferase (hpt), puromycin-N-acetyl-transferase (pac), blasticidin S (bsr), and bleomycin and phleomycin resistance (ble*). These genes are indicated because selection for vectors carrying a particular selectable marker cannot be performed in packaging cells that already express the marker.