Table

Table 1. Choice of matrix for MALDI-MS analysis of different analytes

Matrixa Peptide mappingb Small proteins Large proteins Glyco peptides Glyco proteins References
HCCA 1 3 - 2 - Beavis et al. (1992)
SA - 1 1 3 2 Beavis and Chait (1989)
DHB 2 2 1 1 1 Strupat et al. (1991); Karas et al. (1993)
THAP 2 - - - - Kussman and Roepstorff (2000)
DHAP/DAHC 2 - - - Gorman et al. (1996)
The choice of matrix must be adapted to the physical properties of the analyte. First choice is denoted by 1, second choice by 2, and third choice by 3.
a(HCCA) α-cyano-4-hydroxy-cinnamic acid; (SA) sinapinic acid; (DHB) 2,5-dihyroxybenzoic acid; (THAP) 2,4,6-trihydroxyacetophenone; (DHAP) 2,6-dihydroxyacetophonone; (DAHC) diammonium hydrogen citrate.
bThe choice of matrix can considerably influence the sequence coverage in peptide mapping. For example, peptide mapping data obtained with the matrix THAP can complement those obtained with DHB and HCCA (Kussman and Roepstorff 2000).
(Portion of table adapted from Kussmann and Roepstorff 2000, with permission from Humana Press © 2000.)

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  1. doi:10.1101/pdb.tab194610 Cold Spring Harb Protoc 2008: pdb.tab194610-

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