Recipe

Basic solution

0.5 mM ascorbic acid

0.5 mM CaCl2

10 μM KH2PO4

5 mM MES-Tris (pH 5.5)

0.5 mM MgCl2

Adjust the osmolality to 550 mM/kg with sorbitol.

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  1. doi:10.1101/pdb.rec11358 Cold Spring Harb Protoc 2008: pdb.rec11358-
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