Recipe

Pipette solution for K+ current recordings

10 mM HEPES

20 mM KCl

80 mM potassium glutamate

Before each experiment, add Mg-ATP from a 0.5 M Mg-ATP stock solution stock to a final concentration of 5 mM.

Adjust the osmolality to 550 mM/kg with sorbitol.

Adjust the pH to 7.8 with Tris.

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  1. doi:10.1101/pdb.rec11363 Cold Spring Harb Protoc 2008: pdb.rec11363-

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