Formaldehyde (4%) in PIPES
Formaldehyde (made from freshly depolymerized paraformaldehyde)
0.1 M PIPES buffer (pH 7.4)
Prepare 4% (w/v) formaldehyde in 0.1 M PIPES buffer containing 2 mmol/L CaCl2. CaCl2 is used to enhance the retention of phospholipids during primary fixation.
