Recipe

Formaldehyde (8%) in PIPES

Formaldehyde (made from freshly depolymerized paraformaldehyde)

0.1 M PIPES buffer (pH 7.4)

CaCl2

Prepare 8% (w/v) formaldehyde in 0.1 M PIPES buffer containing 2 mmol/L CaCl2. CaCl2 is used to enhance the retention of phospholipids during primary fixation.

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  1. doi:10.1101/pdb.rec11369 Cold Spring Harb Protoc 2008: pdb.rec11369-
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