Recipe

FISH hybridization solution

50% (v/v) formamide

100 μg/mL heparin

100 μg/mL salmon sperm DNA

5X SSC

0.1% (v/v) Tween 20

Filter through a 0.2-μm filter. The solution is stable for several months stored at -20°C.

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  1. doi:10.1101/pdb.rec11370 Cold Spring Harb Protoc 2008: pdb.rec11370-
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