Formaldehyde (37%, w/v)
MATERIALS
Equipment
Filter, syringe, 45-μm
Fume hood
Hot plate
Stir bar, magnetic
Vial, scintillation, glass
METHOD
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1. Working under a fume hood, in a glass scintillation vial, mix 0.92 g of paraformaldehyde in 2.5 mL of H2O and 35 μL of 1N KOH.
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2. Dissolve by heating on a hot plate while stirring with a magnetic stir bar.
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3. Cool the solution on ice.
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4. Filter through a 0.45-μm syringe filter.
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5. It is best to prepare the solution fresh on the day of tissue fixation, although it may be stored at 4°C for up to 1 wk. The recipe can be scaled up, if necessary.
