Table

Table 1. Statistical parameters for combination of scoring matrices and affine gap penalties

Scoring matrix Gap opening penaltya Gap extension penaltya K λ Hb
BLOSUM50 a 0−∞ 0.110 0.232 0.34
BLOSUM50 15 8−15 0.090 0.222 0.31
BLOSUM50 11 8−11 0.050 0.197 0.21
BLOSUM50 11 1 -- -- --
BLOSUM62 c 0−∞ 0.13 0.318 0.40
BLOSUM62 12 3−12 0.100 0.305 0.38
BLOSUM62 8 7−88 0.060 0.270 0.25
BLOSUM62 7 1 -- -- --
PAM250 a 0−∞ 0.09 0.229 0.23
PAM250 15 5−15 0.060 0.215 0.20
PAM250 10 8−10 0.031 0.175 0.11
PAM250 11 1 -- -- --
Dashes indicate that no value can be calculated because the relationship between alignment score and sequence length is linear and not logarithmic, indicating that the alignment is global, not local, in character. Statistical significance may not be calculated for these gap penalty-scoring matrix combinations. The corresponding values for gap penalties define approximate lower limits that should be used.
aThe penalty for a gap opening of length l is the value of the gap opening penalty shown. The gap extension penalty is not added until the gap length is 2. Make sure that the alignment program uses this same scheme for scoring gaps. The extension penalty is shown over a range of values; values within this range did not change K and λ.
bThe entropy in units of the natural logarithm.
cA value of ∞ for gap penalty will produce alignments with no gaps.

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  1. doi:10.1101/pdb.tab1top40 Cold Spring Harb Protoc 2008: pdb.tab1top40-

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