Recipe

PCR buffer (10X)

40 mM tricine-KOH (pH 9.2 at 22°C)

3.5 mM magnesium acetate

10 mM potassium acetate

75 mg/mL bovine serum albumin (BSA)

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  1. doi:10.1101/pdb.rec11157 Cold Spring Harb Protoc 2008: pdb.rec11157-
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