Recipe

Second-strand buffer (5X)

500 mM KCl

50 mM ammonium sulfate

25 mM MgCl2

0.75 mM β-nicotinamide adenine dinucleotide (β-NAD)

100 mM Tris-Cl (pH 7.5)

0.25 mg/mL bovine serum albumin (BSA)

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  1. doi:10.1101/pdb.rec11182 Cold Spring Harb Protoc 2008: pdb.rec11182-
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