Recipe

EMSA binding buffer (5X)

1 mM DTT (dithiothreitol)

0.1 mM EDTA

25 mM HEPES-KOH, pH 7.6

150 mM NaCl

50 ng/μL poly(dI-dC)

200 ng/μL salmon sperm DNA (sheared)

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  1. doi:10.1101/pdb.rec11378 Cold Spring Harb Protoc 2008: pdb.rec11378-
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