Recipe

Metanil yellow counterstaining solution

0.25%-0.5% (w/v) metanil yellow (C.I. 13065)

0.25%-0.5% (v/v) acetic acid

Combine above reagents. The solution darkens, with deterioration of staining power, after 3 wk.

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  1. doi:10.1101/pdb.rec11383 Cold Spring Harb Protoc 2008: pdb.rec11383-

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