Recipe

Iron alum (5%)

10 g ammonium ferric sulfate (FeNH4[SO4]2•12H2O)

200 mL H2O

Iron alum (ammonium ferric sulfate) should consist of violet crystals that dissolve completely in H2O to make a light orange-brown solution. If the crystals are large, it may be necessary to leave the mixture overnight on a magnetic stirrer. There are many bad batches of this compound, which contain insoluble white or light brown material. Even with clean iron alum, a precipitate forms after several months, and the solution should then be discarded.

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  1. doi:10.1101/pdb.rec11387 Cold Spring Harb Protoc 2008: pdb.rec11387-

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