Recipe

Nuclear fast red solution

0.2 g nuclear fast red (Kernechtrot; C.I. 60760)

10 g aluminum sulfate (Al2[SO4]3•18H2O)

200 mL H2O

Combine above reagents and heat with stirring until nearly boiling, then leave overnight to cool. There is a substantial residue of insoluble material, which must be removed by decanting and filtering. The solution keeps for ~1 yr; it may need to be filtered before each use.

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  1. doi:10.1101/pdb.rec11399 Cold Spring Harb Protoc 2008: pdb.rec11399-

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