Recipe

MBST blocking buffer

 
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MATERIALS

Reagents

Blocking reagent (Roche Applied Science, 11096176001)

Sheep serum (heat-inactivated)

To prepare, incubate stocks of sheep serum for 1 h at 56°C. Aliquot into smaller tubes and store at −20°C.

MBST (1X)

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METHOD

  • 1. Add 2 g of blocking reagent to 100 mL of 1X MBST. Heat and agitate on a magnetic stirrer for 1 h to allow the blocking reagent to go into solution.

  • 2. To prepare 50 mL of blocking buffer, combine 10 mL of heat-inactivated sheep serum with 40 mL of the blocking agent solution from Step 1.

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  1. doi:10.1101/pdb.rec11410 Cold Spring Harb Protoc 2008: pdb.rec11410-
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