Recipe

TNE for SISH

Reagent Amount to add Final concentration
1 M Tris-Cl, pH 7.5 10 mL 10 mM
5 M NaCl 100 mL 500 mM
0.5 M EDTA 2 mL 1 mM
RO-H2O to 1 L
RO-H2O is H2O that has been purified by reverse osmosis (18 MΩ).
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  1. doi:10.1101/pdb.rec11413 Cold Spring Harb Protoc 2008: pdb.rec11413-
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