Recipe

Hybridization buffer for SISH

Reagent Amount to add (for 50 mL) Final concentration
Formamide 25 mL 50% (v/v)
SSC (20X, pH 5) 5 mL 2X (pH 5)
Denhardt’s reagent (50X) 1 mL 1X
Dextran sulfate stock for SISH (50%, w/v) 10 mL 10% (w/v)
tRNA stock (50X) 1 mL 0.2 mg/mL
Salmon sperm DNA stock for SISH (50X) 1 mL 0.5 mg/mL
RO-H2O 7 mL --
RO-H2O is RNase-free H2O that has been purified by reverse osmosis (18 MΩ). All solutions used to make this buffer must be free of RNase activity.

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  1. doi:10.1101/pdb.rec11418 Cold Spring Harb Protoc 2008: pdb.rec11418-
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