Cover image

Cover Illustration: Whole mount in situ hybridization of a 36-h post-fertilization zebrafish embryo with a probe for Zcoe2, the zebrafish homologue of EBF-2, a transcription factor involved in neuronal differentiation. Dorsal view, mounted in 90% glycerol. NBT/BCIP was used as the substrate for the chromogenic reaction. (Image courtesy of Katrin Volkmann and Reinhard Köster, Helmholtz Zentrum München, German Research Center for Environmental Health, Institute of Developmental Genetics, Neuherberg). For more information on this method, see Whole Mount RNA In Situ Hybridization of Zebrafish Embryos: Probe Synthesis (doi:10.1101/pdb.prot5036), Whole Mount RNA In Situ Hybridization of Zebrafish Embryos: Hybridization (doi:10.1101/pdb.prot5037), and Whole Mount RNA In Situ Hybridization of Zebrafish Embryos: Mounting (doi:10.1101/pdb.prot5038).