Whole Mount RNA In Situ Hybridization on Zebrafish Embryos: Mounting

INTRODUCTION

This protocol describes the mounting of zebrafish embryos for observation after whole-mount in situ hybridization. Embryos can be mounted in glycerol or dehydrated and mounted in Permount, with or without the yolk. Glycerol causes less shrinkage, but is not as effective a clearing agent. Embryos mounted on the yolk should be stored in the dark and photographed immediately, because the yolks darken rapidly.