LB agar + carbenicillin plates
LB agar (GIBCO/Invitrogen)
To prepare LB agar plates with 50 μg/mL carbenicillin, combine 32 g of LB agar with 1 L of H2O. Stir and autoclave for 15 min at 121°C. When cooled to 45°C-50°C, add 1 mL of carbenicillin stock solution (50 mg/mL). Pour into Petri dishes and allow to solidify. Store at 4°C.
