Recipe

Physiological buffer (PB)

1 mM dithiothreitol (DTT)

100 mM KCH3COOH

30 mM KCl

1 mM MgCl2

1 mM Na2ATP

10 mM Na2PO4

Adjust pH to 7.4 with 100 mM KH2PO4 if necessary, and add protease and nuclease inhibitors as required.

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  1. doi:10.1101/pdb.rec11419 Cold Spring Harb Protoc 2008: pdb.rec11419-
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