Recipe

Trypsin solution (5%, w/v)

Trypsin, 1:250 (Becton Dickinson, Difco 215240)

Add 5 g trypsin to 100 mL sterile H2O. Refrigerate. Swirl the solution periodically until all of the powder has dissolved. Aliquot the solution into plastic vials (1 mL/vial) and freeze.

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  1. doi:10.1101/pdb.rec11424 Cold Spring Harb Protoc 2008: pdb.rec11424-

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