Recipe

Wright stain

Methanol

Wright stain (Sigma W3000)

Add 1.5 g of Wright stain directly to a fresh 500-mL bottle of methanol. Add a magnetic stirring bar, replace the cap, and stir overnight. Filter the stain, dispensing the filtrate into a dark 500-mL bottle (to protect the stain from light). Store at room temperature.

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  1. doi:10.1101/pdb.rec11426 Cold Spring Harb Protoc 2008: pdb.rec11426-

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