Recipe

Hypotonic solution

Reagent Amount Final concentration
H2O 1 L --
Potassium chloride 5.325 g 71.4 mM
Sodium citrate 0.381 g 1.3 mM
Adjust the pH to 7.2-7.4. Store at 4°C.
This solution is for lymphocytes, amniotic fluid cells, chorionic villus cells, spontaneous abortion tissues, and skin fibroblasts.

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  1. doi:10.1101/pdb.rec11427 Cold Spring Harb Protoc 2008: pdb.rec11427-
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