Recipe

Glycerol (20% in PEM)

20% glycerol (v/v)

80 mM PIPES (Sigma P1851), pH 6.9

1 mM EGTA

1 mM MgCl2

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  1. doi:10.1101/pdb.rec11437 Cold Spring Harb Protoc 2008: pdb.rec11437-
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