Table
Table 2. Sample calculations for determining protein concentration using the BCA assay
| Sample number |
Sample (μL) |
Experiment buffer (μL) |
BCA reagent (mL) |
A562 |
Protein quantity (μg) |
Protein conc. (mg/mL) |
| 1 |
50 |
- |
1 |
>2 |
|
|
| 2 |
50 |
- |
1 |
>2 |
|
|
| 3 |
50 |
- |
1 |
>2 |
|
|
|
|
|
|
Avg = unreliable |
Unreliable |
Unreliable |
|
|
|
|
|
|
|
| 4 |
10 |
40 |
1 |
1.66 |
|
|
| 5 |
10 |
40 |
1 |
1.70 |
|
|
| 6 |
10 |
40 |
1 |
1.65 |
|
|
|
|
|
|
Avg = 1.67 |
83.0 |
8.3 |
|
|
|
|
|
|
|
| 7 |
5 |
45 |
1 |
0.88 |
|
|
| 8 |
5 |
45 |
1 |
0.87 |
|
|
| 9 |
5 |
45 |
1 |
0.87 |
|
|
|
|
|
|
Avg = 0.88 |
43.0 |
8.7 |
|
|
|
|
|
|
|
| 10 |
2.5 |
47.5 |
1 |
0.09 |
|
|
| 11 |
2.5 |
47.5 |
1 |
0.07 |
|
|
| 12 |
2.5 |
47.5 |
1 |
0.09 |
|
|
|
|
|
|
Avg = 0.08 |
Unreliable |
Unreliable |
-
doi:10.1101/pdb.tab294722
Cold Spring Harb Protoc
2008.
2008:
pdb.tab294722-
