Cresyl violet acetate stain

MATERIALS

METHOD

• 1. Add 6 mL of glacial acetic acid to 994 mL H₂O to make Solution A.

• 2. Add 13.6 g of sodium acetate to 1000 mL H₂O to make Solution B.

• 3. Combine Solutions A and B in a 9:1 ratio and titrate to pH 3.7 to make an acetate buffer.

• 4. Combine the acetate buffer with 1% cresyl violet in a 1:1 ratio to make cresyl violet acetate stain.

• 5. Filter the staining solution with an aspirator pump and Whatman #1 filter paper.