Recipe

Selective MS medium

2.5 mM phosphoric acid (H3PO4)

5 mM potassium nitrate (KNO3)

2 mM MgSO4

1 mM Ca(NO3)2

1 mM 2-[N-morpholino]ethanesulfonic acid (MES)

1% (w/v) sucrose

0.7% (w/v) agar type A (adjust pH to 5.7 with KOH)

Add potassium arsenate from a sterile stock solution to the desired final concentration, e.g., 250 μM-2 mM.

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  1. doi:10.1101/pdb.rec11309 Cold Spring Harb Protoc 2008: pdb.rec11309-

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