Recipe

Freezing medium for primary cells

90% fetal bovine serum (FBS) (Hyclone SH30071.03)

10% DMSO (Sigma)

Filter medium with a 0.2-μm syringe filter.

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  1. doi:10.1101/pdb.rec11443 Cold Spring Harb Protoc 2008: pdb.rec11443-
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