Recipe

hFF medium with FBS

90% Iscove’s Modified Dulbecco’s Media (IMDM) with L-glutamine + HEPES (Gibco)

10% fetal bovine serum (FBS; Gibco)

50 U/mL penicillin/streptomycin (Gibco)

Filter medium with a 0.2-μm bottle filter.

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  1. doi:10.1101/pdb.rec11449 Cold Spring Harb Protoc 2008: pdb.rec11449-
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