Recipe

Coimmunoprecipitation buffer (IP-B)

1 mM EDTA

10% (v/v) glycerol

75 mM NaCl

Protease inhibitor cocktail (1 tablet per 10 mL) (Complete, Mini; Roche Applied Science)

0.05% (w/v) SDS

100 mM Tris-Cl (pH 7.4)

0.1% (v/v) Triton X-100

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  1. doi:10.1101/pdb.rec11460 Cold Spring Harb Protoc 2008: pdb.rec11460-
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