Recipe

Nuclei lysing buffer

0.1 mg/mL 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF)

10 mM EDTA

50 mM HEPES (pH 8)

Protease inhibitor cocktail (one tablet per 10 mL) (Complete, Mini; Roche Applied Science)

1% (w/v) SDS

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  1. doi:10.1101/pdb.rec11467 Cold Spring Harb Protoc 2008: pdb.rec11467-
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